If 750 mg of GABA (Gamma-Aminobutyric acid were ingested 3 times a day,
along with B6, would it cross the blood-brain barrier? I’ve heard that
it helps with anxiety.
Frank
—
19
Apr
GABA
posted by admin in Uncategorized and have Comment (1)
In article <CoAzJw….@csug.cs.reading.ac.uk>, afsmi…@csug.cs.reading.ac.uk (Luke A. D. Miller) writes:
>I am currently working on a project involved in the development of a
>method for the detection of adulteration of edible oils such as extra
>virgin olive oil.
You will find there is a huge amount of literature on this, especially
using chromatographic techniques. If you look in the Journal of the
American Oil Chemists Association you should find some interesting
papers.
>My chosen route for this detection is the isolation and analysis of the
>sterol esters present at about 1% in the oil since the content and
> composition of these compounds has been shown to be specific to
> a particular oil.
Good luck, I would suggest that they will vary significantly according
to how the oil is processed.
>I have carried out a literature review, but would be interested in
> hearing from anyone with useful comments w.r.t. the following:
>1. Isolation of intact sterol esters from oil
I’ve analysed them directly in lipids without separating them from the
triglyceride using an 8 meter x 0.10mm ID x 0.1um Durabond 1 capillary
with H2 carrier and FID. I’ve also separated them using a silica
SepPak and chloroform/ methanol – but there are lots of methods.
Check out Lipid Analysis by W.W.Cristie. It’s an excellent book.
>2. Effects of refining, particularly steam deoderisation, on sterol esters
This is where the above journal will help. Your literature search
should have found heaps, as there have been lots of attempts to find
a marker for adulteration. I understand that there was a recent
paper demonstrating some of the newer adulteration techniques
virtually matched the oil to the virgin oil composition, suggesting
the adulterating companies were using more exotic analytical techniques
than the regulators. Unfortunately I can’t remember where I read the
discussion, it could have been in JAOC or a general purpose mag like
New Scientist. If it’s really important I’ll try and find it, but
your search should have hit it.
>Thank you in anticipation
Bruce Hamilton
Bruce Hamilton